Category (Product Type)

Kit

UNSPSC

41116158

Shipping Temp

Room Temp

Product Line

Oxidative Stress/Damage

Product feature 3

Unknown samples are compared with a provided standard

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Product feature 1

Detect as little as 50 nM of ethenoadenosine or 625 nM of ethenocytidine from DNA isolated from cells or tissues

URL link to SDS

(STA-820-C) GHS.pdf

Product feature 2

Ethenoadenosine and ethenocytidine are assayed in separate wells; each kit can perform a combined total of 96 assays in any ratio of the two targets

Description

Induced protein genes, factors or kinases, increase the production of (an enzyme or other protein) at the level of genetic transcription.

Storage Temp

Upon receipt, store the Ethenoadenosine Standard, Ethenocytidine Standard, Anti-Ethenoadenosine Antibody and Anti-Ethenocytidine Antibody at -20ºC. Store all other components at 4ºC until their expiration dates.

Short Description

The OxiSelect™ Aldehyde-Induced DNA Damage ELISA Combo Kit (Ethenoadenosine / Ethenocytidine Quantitation) is a competitive ELISA kit allowing the quantitation of ethenoadenosine or ethenocytidine adducts in DNA isolated from cells or tissues. Samples and standards are added to a provided microplate preabsorbed with etheno-damaged DNA. A primary antibody is added and binds competitively to ethenoadenosine in the unknown sample or to the etheno adduct in the DNA coated on the plate. After washing a secondary antibody is added. A low signal means most of the primary antibody was bound to the unknown, indicating a high level of etheno adducts in the sample. Each combo kit can perform up to 96 assays combined for ethenoadenosine and ethenocytidine. The two targets are assayed in separate wells, but any well on the provided plate may be used for either target in any ratio (e.g. 64 wells for ethenoadenosine and 32 wells for ethenocytidine, or 48 wells for each).