Stability
NA
Precision
NA
Specificity
NA
Target Name
NA
Species Reactivity
Human
Assay Type
Sandwich
Shipping Conditions
Ice packs
Target's alterntive name
DNase-Ⅰ
Sensitivity
0.1 ng/mL
Assay Time
4.5 hours
Detection Method
Colorimetric
Detection Range
0.16-10 ng/mL
Estimated Turnaround Time
6-11 business days
Sample Type
Serum, Plasma, Biological Fluids
Storage Temperature
Short term: 4°C; Long term: see manual.
Application
For research use only. Not for diagnostic procedures.
Quality Systems
The kit is manufactured at ISO 9001 certified facilities.
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Cross Activity
No significant cross-reactivity or interference was observed.
Precaution of Use
The Stop Solution is acidic. Do not allow to contact skin or eyes.
Research Area
Cancer, Cell Biology, Epigenetics and Nuclear Signaling, Metabolism
Shelf life
Use Human DNase-Ⅰ (Deoxyribonuclease Ⅰ) ELISA Kit before 6 months
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Test Principle
This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human DNase-I . Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human DNase-I and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human DNase-I, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human DNase-I. The concentration of Human DNase-I in samples can be calculated by comparing the OD of the samples to the standard curve.
