Assay Time

3 hours

Shipping Conditions

Ice packs

Sensitivity

1.44 ng/mL

Detection Method

Colorimetric

Detection Range

3.12-200 ng/mL

Estimated Turnaround Time

6-11 Business days

Target Name

Complement Factor B

Species Reactivity

Homo sapiens (Human)

Assay Type

Double-antibody Sandwich

Storage Temperature

Short term: 4°C; Long term: see manual.

Sample Type

Serum, Plasma and other biological fluids

Application

For research use only. Not for diagnostic procedures.

Research Area

Infection immunity, Immune molecule, Hematology, Autoimmunity

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Precaution of Use

The Stop Solution is acidic. Do not allow to contact skin or eyes.

Quality Systems

The kit is manufactured at ISO 9001 and ISO 13485 certified facilities.

Shelf life

Use Wide-range Human Complement Factor B (CFB) ELISA Kit before 8 months

Specificity

This assay has high sensitivity and excellent specificity for detection of Complement Factor B

Test Principle

No significant cross-reactivity or interference between Complement Factor B and analogues was observed.

Cross Activity

No significant cross-reactivity or interference between Complement Factor B and analogues was observed.

Target's alterntive name

CF-B, BF, BFD, CFAB, GBG, H2-Bf, PBF2, B-Factor, Properdin, Properdin factor B, C3/C5 convertase, Glycine-rich beta glycoprotein

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Complement Factor B were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Complement Factor B were tested on 3 different plates, 8 replicates in each plate.CV (%) = SD/meanX100;Intra-Assay: CV

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Description

Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.Low range, broad range, wide range or standard range protein kDa markers or DNA bp-kb ladders are available from Biomatik. ELISA kits in the standard range can be adapted to the high sensitive range only by adding some points to the lower end of the standard curve.