Assay Time
3 hours
Sensitivity
27 pg/mL
Shipping Conditions
Ice packs
Detection Method
Colorimetric
Detection Range
78.12-5000 pg/mL
Latin name
Rattus norvegicus
Estimated Turnaround Time
6-11 Business days
Species Reactivity
Rattus norvegicus (Rat)
Assay Type
Double-antibody Sandwich
Target Name
O-6-Methylguanine DNA Methyltransferase
Storage Temperature
Short term: 4°C; Long term: see manual.
Sample Type
Tissue homogenates and other biological fluids.
Research Area
Signal transduction, Enzyme & Kinase, Tumor immunity
Application
For research use only. Not for diagnostic procedures.
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Precaution of Use
The Stop Solution is acidic. Do not allow to contact skin or eyes.
Quality Systems
The kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
Shelf life
Use Rat O-6-Methylguanine DNA Methyltransferase (MGMT) ELISA Kit before 8 months
Specificity
This assay has high sensitivity and excellent specificity for detection of O-6-Methylguanine DNA Methyltransferase
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Cross Activity
No significant cross-reactivity or interference between O-6-Methylguanine DNA Methyltransferase and analogues was observed.
Test Principle
No significant cross-reactivity or interference between O-6-Methylguanine DNA Methyltransferase and analogues was observed.
Target's alterntive name
Methylated-DNA--Protein-Cysteine Methyltransferase, 6-O-methylguanine-DNA methyltransferase, Methylated-DNA--protein-cysteine methyltransferase
About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level O-6-Methylguanine DNA Methyltransferase were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level O-6-Methylguanine DNA Methyltransferase were tested on 3 different plates, 8 replicates in each plate.CV (%) = SD/meanX100;Intra-Assay: CV
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
