Assay Time
3 hours
Shipping Conditions
Ice packs
Sensitivity
0.060 ng/mL
Detection Method
Colorimetric
Detection Range
0.156-10 ng/mL
Estimated Turnaround Time
6-11 Business days
Species Reactivity
Homo sapiens (Human)
Assay Type
Double-antibody Sandwich
Target Name
O-6-Methylguanine DNA Methyltransferase
Storage Temperature
Short term: 4°C; Long term: see manual.
Research Area
Signal transduction, Enzyme & Kinase, Tumor immunity
Application
For research use only. Not for diagnostic procedures.
Sample Type
Tissue homogenates, Cell lysates and other biological fluids
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Precaution of Use
The Stop Solution is acidic. Do not allow to contact skin or eyes.
Quality Systems
The kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
Shelf life
Use Human O-6-Methylguanine DNA Methyltransferase (MGMT) ELISA Kit before 8 months
Specificity
This assay has high sensitivity and excellent specificity for detection of O-6-Methylguanine DNA Methyltransferase
Cross Activity
No significant cross-reactivity or interference between O-6-Methylguanine DNA Methyltransferase and analogues was observed.
Test Principle
No significant cross-reactivity or interference between O-6-Methylguanine DNA Methyltransferase and analogues was observed.
Target's alterntive name
Methylated-DNA--Protein-Cysteine Methyltransferase, 6-O-methylguanine-DNA methyltransferase, Methylated-DNA--protein-cysteine methyltransferase
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level O-6-Methylguanine DNA Methyltransferase were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level O-6-Methylguanine DNA Methyltransferase were tested on 3 different plates, 8 replicates in each plate.CV (%) = SD/meanX100;Intra-Assay: CV
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
