Assay Time
3 hours
Shipping Conditions
Ice packs
Sensitivity
0.054 ng/mL
Detection Method
Colorimetric
Detection Range
0.156-10 ng/mL
Estimated Turnaround Time
6-11 Business days
Species Reactivity
Homo sapiens (Human)
Target Name
Heat Shock Protein 40
Assay Type
Double-antibody Sandwich
Storage Temperature
Short term: 4°C; Long term: see manual.
Research Area
Signal transduction, Developmental science
Application
For research use only. Not for diagnostic procedures.
Sample Type
Tissue homogenates, Cell lysates and other biological fluids
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Shelf life
Use Human Heat Shock Protein 40 (HSP40) ELISA Kit before 8 months
Precaution of Use
The Stop Solution is acidic. Do not allow to contact skin or eyes.
Quality Systems
The kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
Specificity
This assay has high sensitivity and excellent specificity for detection of Heat Shock Protein 40
Cross Activity
No significant cross-reactivity or interference between Heat Shock Protein 40 and analogues was observed.
Test Principle
No significant cross-reactivity or interference between Heat Shock Protein 40 and analogues was observed.
Target's alterntive name
DnaJ, DNAJB1, Sis1, RSPH16B, HSPF1, DnaJ homolog subfamily B member 1, Radial Spoke 16 Homolog B, Heat Shock 40kDa Protein 1, DnaJ protein homolog 1
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Heat Shock Protein 40 were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Heat Shock Protein 40 were tested on 3 different plates, 8 replicates in each plate.CV (%) = SD/meanX100;Intra-Assay: CV
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
