Assay Time
3 hours
Shipping Conditions
Ice packs
Sensitivity
0.064 ng/mL
Detection Method
Colorimetric
Detection Range
0.156-10 ng/mL
Latin name
Rattus norvegicus
Estimated Turnaround Time
6-11 Business days
Research Area
Signal transduction
Species Reactivity
Rattus norvegicus (Rat)
Assay Type
Double-antibody Sandwich
Storage Temperature
Short term: 4°C; Long term: see manual.
Target Name
Growth Arrest And DNA Damage Inducible Protein Alpha
Application
For research use only. Not for diagnostic procedures.
Target's alterntive name
DDIT1, GADD45, DNA damage-inducible transcript 1 protein
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Sample Type
Tissue homogenates, Cell lysates and other biological fluids.
Precaution of Use
The Stop Solution is acidic. Do not allow to contact skin or eyes.
Quality Systems
The kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
Shelf life
Use Rat Growth Arrest And DNA Damage Inducible Protein Alpha (GADD45a) ELISA Kit before 8 months
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Specificity
This assay has high sensitivity and excellent specificity for detection of Growth Arrest And DNA Damage Inducible Protein Alpha
Description
The Growth Arrest DNA Damage Protein Alpha (GADD45a) ELISA Kit is a α- or alpha protein sometimes glycoprotein present in blood.
Cross Activity
No significant cross-reactivity or interference between Growth Arrest And DNA Damage Inducible Protein Alpha and analogues was observed.
Test Principle
No significant cross-reactivity or interference between Growth Arrest And DNA Damage Inducible Protein Alpha and analogues was observed.
About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Growth Arrest And DNA Damage Inducible Protein Alpha were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Growth Arrest And DNA Damage Inducible Protein Alpha were tested on 3 different plates, 8 replicates in each plate.CV (%) = SD/meanX100;Intra-Assay: CV
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
