Verified reactivity
Rat
Verified applications
ELISA
Shipping requirements
Blue ice
Use for
6 months
Assay duration
1-5 hours
Sensitivity limit
0,078 ng/ml
ELISA detection
Colorimetric
Assay class
Please contact us
Latin name
Rattus norvegicus
Estimated production time
7-11 business days
Detection limits
0.312 ng/ml-20 ng/ml
Protein number
Please refer to Uniprot
Gene number
Please refer to GenBank
Alternate protein number
Please refer to SwissProt
Research main area
Epigenetics and Nuclear Signaling
Samples to be analyzed
serum, plasma, tissue homogenates, cell lysates
Gene name
replication protein A 70 kDa DNA-binding subunit
ELISA's stability
Please see ELISA's datasheet, otherwise contact us
Precision of the test
Please see ELISA's datasheet, otherwise contact us
Protocol
Please see ELISA's datasheet, otherwise contact us
Assay principle
Please see ELISA's datasheet, otherwise contact us
Notes
For research use only. Not for diagnostic procedures.
QC
The kit is manufactured at ISO 9001 certified facilities.
ELISA's specificity
To know the specificity of this test please feel free to contact us
Alternate gene name
HSSB, MST075, REPA1, RF-A, RP-A, RPA70, MSTP075|replication protein A1
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
ELISA's cross-reactivity
This assay doesn't seem to cross-react with other species. For more information about cross-reactivity please contact us.
Storage recommendation
-20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
Warnings
The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED,Replication factor subunits and DNA replication proteins are found in DNA replication complexes like GINS, MCM,.. and replication initiators producing two identical replicas of DNA from one original DNA molecule. This process occurs in all living organisms and is the basis for biological inheritance. DNA is made up of a double helix of two strands, and each strand of the original DNA molecule gene serves as a template for the production of the complementary strand, a process referred to as semiconservative replication. Cellular proofreading and error-checking mechanisms ensure near perfect fidelity for DNA replication of genes.
Description
The kilo Daltons subunit weight of protein A 70 DNA-binding subunit (RPA1) ELISA Kit, 96 compared to your protein ladder can be shifted a little due to electrophoresis effects. 1 kDa = 1000 g/mol protein,A microtiter plate (spelled Microtiter is a registered trade name in the United States) or microplate or micro well plate or multiwell, is a flat plate with multiple "wells" used as small test tubes. The microplate has become a standard tool in analytical research and clinical diagnostic testing laboratories. A very common usage is in the enzyme-linked immunosorbent assay (ELISA), the basis of most modern medical diagnostic testing in humans and animals.
A microplate typically has 6, 24, 96, 384 or 1536 sample wells arranged in a 23 rectangular matrix. Some microplates have even been manufactured with 3456 or 9600 wells, and an "array tape" product has been developed that provides a continuous strip of microplates embossed on a flexible plastic tape.
