Tested for

ELISA

Shipping conditions

Blue Ice

Area of research

DNA & RNA

Category

Research kit

Storage

Store at 2-8 deg C

Usage Recommendations

Optimal conditions to be determined by end-user

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

Assay Information

AntissDNA is an indirect solid phase enzyme immunometric assay (ELISA). It is designed for the quantitative measurement of IgG class autoantibodies directed against singlestranded DNA. The assay is based on microplates coated with recombinant singlestranded DNA. The microplate can be divided into 12 modules of 8 wells each or can be used complete for 96 determinations. During this procedure the binding of present autoantibodies, as well as the formation of the sandwich complexes and enzymatic color reaction take place during three different reaction phases. The amount of colour is directly proportional to the concentration of IgG present in the original sample. The optical density of the controls serve as reference for the determination of the cutoff OD. Patent results may be classified in terms of negative, positive or strong positive. Alternatively the concentrations of the controls may be used to calculate a calibration curve for semiquantitative results.