This reagent is purified
0.97
Species compatible to
Humans
Use of this test kit or product
Genetics lab
Living modified organisme in this reagent or LMO
Not applicable
We send this reagant on
Room temperature
Molecular weight
in kilo Danlto or kDa
Type of reagent
Oxidative Stress/Damage
Antigen
a specific protein sequence
Group
Enzyme-Linked Immunosorbent Assay kits
Reagent properties
Detection sensitivity of 150 nM 5MedCyd
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Protocol information
Suitable for use with DNA samples such as cell or tissue genomic DNA
Cellular use
For detection and quantification of 5'-methyl-2'-deoxycytidine (5MedCyd)
Buffer
The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,37
Related techniques
Oxidative Stress/ Damage, DNA / RNA Damage and Repair, Global DNA Methylation and Hydroxymethylation
SDS or safty data sheet
(STA-380) GHS.pdf
The temperatureerature for storage is
Upon receipt, aliquot and store the 5MedCyd Standard and 5MedCyd DNA Conjugate at -20ºC to avoid multiple freeze/thaw cycles. Store all other components at 4ºC.
Storage
Human ELISAs or ELISA kits will be stored at +4°C. The expiry date is mostly determined by the standard stability. If your ELISA kit is expired we can supply a new standard so it will still be functional.
Use of the reagent
The Global DNA Methylation ELISA test kit is a competitive ELISA for the quantitative measurement of 5-methylcytosine (5MedCyd). The unknown 5MedCyd samples or 5MedCyd standards are first added to a 5MedCyd DNA conjugate coated EIA plate. After a brief incubation, an anti-5MedCyd monoclonal antibody is added, followed by an HRP conjugated secondary antibody. The 5MedCyd content in unknown samples is determined by comparison with a predetermined 5MedCyd standard curve.
Gene
Non-methylated DNA at CpG islands organizes histone methylation through ZF-CxxC proteins in epigenetic biological systems. DNA and histone Methylation is catalyzed by enzymes; such methylation can be involved in hodyonr modification, regulation of gene expression, regulation of protein function, and RNA processing. Methylation of heavy metals can also occur outside biological systems. Chemical methylation of tissue samples is also one method for reducing certain histological staining artifacts. The counterpart of methylation is demethylation.
Description
A methyl group is an alkyl derived from methane, containing one carbon atom bonded to three hydrogen atoms — CH3. In formulas, the group is often abbreviated Me. Such hydrocarbon groups occur in many organic compounds. It is a very stable group in most molecules. While the methyl group is usually part of a larger molecule, it can be found on its own in any of three forms anion, cation or radical. The anion has eight valence electrons, the radical seven and the cation six. All three forms are highly reactive and rarely observed,
