This reagent is purified
0.97
Species compatible to
Humans
We send this reagant on
Blue Ice
Use of this test kit or product
Genetics lab
Molecular weight
in kilo Danlto or kDa
Type of reagent
Oxidative Stress/Damage
Protocol information
BPDE-DNA standard included
Antigen
a specific protein sequence
Group
Enzyme-Linked Immunosorbent Assay kits
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Reagent properties
Suitable for use with DNA samples such as cell or tissue genomic DNA
Buffer
The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,19
Related techniques
Oxidative Stress/ Damage, DNA / RNA Damage and Repair, BPDE DNA Adduct ELISA
Cellular use
For detection and quantification of benzo(a)pyrene diol epoxide (BPDE) DNA adducts
SDS or safty data sheet
(STA-357) GHS.pdf
The temperatureerature for storage is
Upon receipt, aliquot and store the Reduced DNA and BPDE-DNA Standards at -20ºC to avoid multiple freeze/thaw cycles. Store all other test kit components at 4ºC.
Storage
Human ELISAs or ELISA kits will be stored at +4°C. The expiry date is mostly determined by the standard stability. If your ELISA kit is expired we can supply a new standard so it will still be functional.
Use of the reagent
BPDE-DNA standards or unknown DNA samples are adsorbed onto a 96-well DNA high-binding plate. The BPDE-DNA adducts present in the sample or standard are probed with an Anti-BPDE-I Antibody, followed by an HRP Conjugated Secondary Antibody. The BPDE-DNA adduct content in an unknown sample is determined by comparing with a standard curve that is prepared from predetermined BPDE-DNA standards.
