Assay procedure
1-4h
Species reactivity
Human
Detection wavelengt
450 nm
Antigen
DNA replication factor Cdt1
Applications
Enzyme Linked Immunosorbent Assay
Synonym name
Homo sapiens DNA replication factor Cdt1 ELISA Kit
Long name
ELISA test kit for Human DNA replication factor Cdt1
General description
This is a ready to use kit for performing ELISA tests for detection of DNA replication factor Cdt1 in samples from Human
Sample types
Our DNA replication factor Cdt1 ELISA kit has been proven to work and successfully detect DNA replication factor Cdt1 in Human
Shipping, storage, shelf life
The DNA replication factor Cdt1 ELISA kit is shipped on ice packs. Upon delivery store the kit at +4 C. The shelf life of the Human DNA replication factor Cdt1 ELISA Kit is 6 months after delivery.
Kit contents
ELISA test kit for Human DNA replication factor Cdt1Assay plate 1 (96wells); Standard 5 x 0.5 ml; A ntibody 1x5ml; Conjugate 1x10ml; Wash buffer 20ml (25X); Substrate A 1x5ml; Substrate B 1x5ml; Stop solution 1x5ml;Adhesive strips (4pcs); working manual
Description
Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Tips
Due to transportation and handling small amounts of the liquid components of the Homo sapiens DNA replication factor Cdt1 ELISA Kit can get caught on the walls or lid of the vial. Prior to use be sure to briefly centrifuge all vials to ensure that all of the content is on the bottom. Do not freeze the kit, as this might denaturate the standards and the peptide chains of the antibodies used for the production if the ELISA kit. Substrate A and Substrate B are light sensitive, therefore you should protect the kits from direct light. When using the vials of Substrates A and B you may cover them in aluminium foil to protect them from light influences.
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED,Replication factor subunits and DNA replication proteins are found in DNA replication complexes like GINS, MCM,.. and replication initiators producing two identical replicas of DNA from one original DNA molecule. This process occurs in all living organisms and is the basis for biological inheritance. DNA is made up of a double helix of two strands, and each strand of the original DNA molecule gene serves as a template for the production of the complementary strand, a process referred to as semiconservative replication. Cellular proofreading and error-checking mechanisms ensure near perfect fidelity for DNA replication of genes.
Summary of the prodecure
1. Prepare the reagents and samples according to the manual; 2. Leave one well empy; 3. Place 50ul of standard or sample in each well; 4. Add 50ul of the antibody to each well except the Blank (empty); 5 Allow 60 minutes for incubation at 37 degrees celsius; 6. Wash the wells 5 times; 7. Add 100ul of the conjugate to each well but the Blank one; 8. Allow 30 more minutes for incubation at 37C; 9. Wash 5 more times; 10. Add 50ul of both Substrate A and Substrate B in each well (incl. the Blank) and incubate for 15 minutes; 11. Put 50 ul of Stop Solution in all wells (incl. the Blank) and read the results within maximum 10 minutes; Notes: *Samples might require dilution, follow the sample dilution instructions of the manual. **During the last incubation with Substrates A and B the setting should be protected from any light.
