Additional isotype
IgG
Assay procedure
1-4h
Species reactivity
Human
Detection wavelengt
450 nm
French translation
anticorps
Applications
Enzyme Linked Immunosorbent Assay
Antigen
anti-double stranded DNA(dsDNA) antibody(IgG)
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Synonym name
Homo sapiens anti-double stranded DNA(dsDNA) antibody(IgG) ELISA Kit
Long name
ELISA test kit for Human anti-double stranded DNA(dsDNA) antibody(IgG)
General description
This is a ready to use kit for performing ELISA tests for detection of anti-double stranded DNA(dsDNA) antibody(IgG) in samples from Human
Sample types
Our anti-double stranded DNA(dsDNA) antibody(IgG) ELISA kit has been proven to work and successfully detect anti-double stranded DNA(dsDNA) antibody(IgG) in Human
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Shipping, storage, shelf life
The anti-double stranded DNA(dsDNA) antibody(IgG) ELISA kit is shipped on ice packs. Upon delivery store the kit at +4 C. The shelf life of the Human anti-double stranded DNA(dsDNA) antibody(IgG) ELISA Kit is 6 months after delivery.
Kit contents
ELISA test kit for Human anti-double stranded DNA(dsDNA) antibody(IgG)Assay plate 1 (96wells); Standard 5 x 0.5 ml; A ntibody 1x5ml; Conjugate 1x10ml; Wash buffer 20ml (25X); Substrate A 1x5ml; Substrate B 1x5ml; Stop solution 1x5ml;Adhesive strips (4pcs); working manual
About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
Properties
If you buy Antibodies supplied by SAB ELISA KIT they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Tips
Due to transportation and handling small amounts of the liquid components of the Homo sapiens anti-double stranded DNA(dsDNA) antibody(IgG) ELISA Kit can get caught on the walls or lid of the vial. Prior to use be sure to briefly centrifuge all vials to ensure that all of the content is on the bottom. Do not freeze the kit, as this might denaturate the standards and the peptide chains of the antibodies used for the production if the ELISA kit. Substrate A and Substrate B are light sensitive, therefore you should protect the kits from direct light. When using the vials of Substrates A and B you may cover them in aluminium foil to protect them from light influences.
Summary of the prodecure
1. Prepare the reagents and samples according to the manual; 2. Leave one well empy; 3. Place 50ul of standard or sample in each well; 4. Add 50ul of the antibody to each well except the Blank (empty); 5 Allow 60 minutes for incubation at 37 degrees celsius; 6. Wash the wells 5 times; 7. Add 100ul of the conjugate to each well but the Blank one; 8. Allow 30 more minutes for incubation at 37C; 9. Wash 5 more times; 10. Add 50ul of both Substrate A and Substrate B in each well (incl. the Blank) and incubate for 15 minutes; 11. Put 50 ul of Stop Solution in all wells (incl. the Blank) and read the results within maximum 10 minutes; Notes: *Samples might require dilution, follow the sample dilution instructions of the manual. **During the last incubation with Substrates A and B the setting should be protected from any light.
