Assay length
3h
Standard
10ng/mL
Assay Type
Sandwich
Sensitivity
0.064ng/mL
Detection range
0.156-10ng/mL
Latin name
Rattus norvegicus
Research Area
Signal transduction;
Alternative Names
DDIT1; GADD45; DNA damage-inducible transcript 1 protein
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Description
The GADD45a(Growth Arrest DNA Damage Protein Alpha) ELISA Kit is a α- or alpha protein sometimes glycoprotein present in blood.
About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Growth Arrest And DNA Damage Inducible Protein Alpha (GADD45α). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Growth Arrest And DNA Damage Inducible Protein Alpha (GADD45α). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Growth Arrest And DNA Damage Inducible Protein Alpha (GADD45α), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Growth Arrest And DNA Damage Inducible Protein Alpha (GADD45α) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
