Assay length

3h

Standard

10ng/mL

Assay Type

Sandwich

Sensitivity

0.059ng/mL

Detection range

0.156-10ng/mL

Research Area

Signal transduction;

Alternative Names

DDIT1; GADD45; DNA damage-inducible transcript 1 protein

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Description

The GADD45a(Growth Arrest DNA Damage Protein Alpha) ELISA Kit is a α- or alpha protein sometimes glycoprotein present in blood.

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Growth Arrest And DNA Damage Inducible Protein Alpha (GADD45α). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Growth Arrest And DNA Damage Inducible Protein Alpha (GADD45α). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Growth Arrest And DNA Damage Inducible Protein Alpha (GADD45α), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Growth Arrest And DNA Damage Inducible Protein Alpha (GADD45α) in the samples is then determined by comparing the O.D. of the samples to the standard curve.