Gene
MCM2
Detection at
450 nm
Sensitivity
6.25 pg/mL
Latin name
Mus musculus
Detection Metod
Sandwich EIA
Specias
Mouse (Mus musculus)
Required sample
Between 1 and 200 µl
Long name
Mouse DNA replication licensing factor MCM2
Sampe type
blood, serum, plasma, other biological fluids
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Description
Mouse DNA replication licensing factor MCM2 (MCM2) ELISA Kitis manufactured by highest quality antibodies and plates to provide you with excellent and reproducible results in your work.It detects with high sensitivity of 6.25 pg/mL. No segnificant cross referance was observed.
Components of the kit
The kit contains 1X plate of 48 wells as well as lyophilized standard. Biotin-Conjugate and Streptavidin-HRP that has to be deluted 100 times. The wash buffer that has to be deluted 25 times. All the required delutions for the preparation of the solutions are also present in the ELISA Kit.
Storage and handing
Mouse DNA replication licensing factor MCM2 (MCM2) ELISA Kit recommended temperature for storage is 4° C . Stored proparly at temperature between 2 - 8°C the ELISA kit can be used witin the expiration date( less than 5% loss rate). Inproper storage at temperature of 37°C can decreese the live of the kit significantly.
Additional description
Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED,Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.Replication factor subunits and DNA replication proteins are found in DNA replication complexes like GINS, MCM,.. and replication initiators producing two identical replicas of DNA from one original DNA molecule. This process occurs in all living organisms and is the basis for biological inheritance. DNA is made up of a double helix of two strands, and each strand of the original DNA molecule gene serves as a template for the production of the complementary strand, a process referred to as semiconservative replication. Cellular proofreading and error-checking mechanisms ensure near perfect fidelity for DNA replication of genes.