Assay Time
3 hours
Shipping Conditions
Ice packs
Research Area
Apoptosis
Sensitivity
0.052 ng/mL
Detection Method
Colorimetric
Detection Range
0.156-10 ng/mL
Estimated Turnaround Time
6-11 Business days
Species Reactivity
Homo sapiens (Human)
Target Name
Caspase Activated DNase
Assay Type
Double-antibody Sandwich
Storage Temperature
Short term: 4°C; Long term: see manual.
Sample Type
Tissue homogenates and other biological fluids.
Application
For research use only. Not for diagnostic procedures.
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Shelf life
Use Human Caspase Activated DNase (CAD) ELISA Kit before 8 months
Precaution of Use
The Stop Solution is acidic. Do not allow to contact skin or eyes.
Quality Systems
The kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
Specificity
This assay has high sensitivity and excellent specificity for detection of Caspase Activated DNase
Cross Activity
No significant cross-reactivity or interference between Caspase Activated DNase and analogues was observed.
Test Principle
No significant cross-reactivity or interference between Caspase Activated DNase and analogues was observed.
Target's alterntive name
DFF-B, DFFB, CPAN, DFF40, DFF2, DNA Fragmentation Factor 40kDa Beta Polypeptide, Caspase-activated deoxyribonuclease, Caspase-activated nuclease
Description
Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Caspase Activated DNase were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Caspase Activated DNase were tested on 3 different plates, 8 replicates in each plate.CV (%) = SD/meanX100;Intra-Assay: CV
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
