Bovine Deoxyribonuclease I (DNASE1) ELISA Kit, 96 well plate

Order Bovine Deoxyribonuclease I DNASE1 ELISA Kit 96 well plate 01012505417 at Gentaur Deoxyribonuclease I (DNASE1) 96


Bovine Deoxyribonuclease I (DNASE1) ELISA Kit, 96 well plate



Catalog no.



961 EUR



Verified applications


Assay duration

3 hours

Assay class


Shipping requirements

Blue ice

Use for

8 months

Sensitivity limit

0,62 ng/mL

ELISA detection


Detection limits

1.56-100 ng/mL

Estimated production time

7-11 business days

Gene name

Deoxyribonuclease I

Protein number

Please refer to Uniprot

Gene number

Please refer to GenBank

Alternate protein number

Please refer to SwissProt

Verified reactivity

Bos taurus; Bovine (Cattle)

Research main area

Enzyme & Kinase;Developmental science;

Alternate gene name

DNaseI; DNL1; DRNI; DNase-I; Dornase alfa


Please see ELISA's datasheet, otherwise contact us


For research use only. Not for diagnostic procedures.

Samples to be analyzed

Serum, plasma, tissue homogenates and other biological fluids


ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED


The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.


E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

ELISA's cross-reactivity

This assay doesn't seem to cross-react with other species. For more information about cross-reactivity please contact us.

ELISA's specificity

This assay has high sensitivity and excellent specificity for detection of Deoxyribonuclease I (DNASE1). No significant cross-reactivity or interference between Deoxyribonuclease I (DNASE1) and analogues was observed.

Storage recommendation

-20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.


The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Precision of the test

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Deoxyribonuclease I (DNASE1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Deoxyribonuclease I (DNASE1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV

ELISA's stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.


A microtiter plate (spelled Microtiter is a registered trade name in the United States) or microplate or micro well plate or multiwell, is a flat plate with multiple "wells" used as small test tubes. The microplate has become a standard tool in analytical research and clinical diagnostic testing laboratories. A very common usage is in the enzyme-linked immunosorbent assay (ELISA), the basis of most modern medical diagnostic testing in humans and animals. A microplate typically has 6, 24, 96, 384 or 1536 sample wells arranged in a 23 rectangular matrix. Some microplates have even been manufactured with 3456 or 9600 wells, and an "array tape" product has been developed that provides a continuous strip of microplates embossed on a flexible plastic tape.

Assay principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Deoxyribonuclease I (DNASE1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Deoxyribonuclease I (DNASE1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Deoxyribonuclease I (DNASE1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Deoxyribonuclease I (DNASE1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.